LOCUS       GLB1L3-pGEc2            7185 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(6663..6792)
                     /note="3'-ITR"
     misc_feature    complement(6361..6575)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    5813..6354
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    4992..5014
                     /note="attB2"
     misc_feature    5792..5797
                     /note="BstB1 site"
     misc_feature    5015..5710
                     /note="Super GFP"
     misc_feature    5711..5761
                     /note="AviTag"
     misc_feature    5762..5785
                     /note="His Tag"
     PCR_frame       3011..4991
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    3011..4969
                     /note="OPTIMIZED GLB1L3 coding region"
     misc_feature    4970..4991
                     /note="TEV"
     vector          join(5000..7185,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..4999
                     /source="GLB1L3 pDONR"
                     /type="Entry Clone"
     source          1..7185
                     /dnas_title="GLB1L3-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGAAGTCCC CACCTCTGCT GTCTCCATGC CTGAGCTGGA AGAGAATGGC
     3061 CGGCATCTTC TTTCTGCCCT TCATCTCCAG CGGCTTCGCC CCCAGATTCA AGCAGGAAGA
     3121 AAACTTCATG CTGGGCAGGG CCCACCCCAG CCAGCCTAGA TTCAATTGGA GCCACCTGAC
     3181 CCCCCTGGAA CTGAAGAATA GATCTGTGGG CCTGGGCACC GAGAGCACAG GCAGAGGAAA
     3241 ACCTCACTTC ACCCTGGAAG GCCACAAGTT TCTGATCTTC GGCGGCAGCA TCCACTACTT
     3301 CAGAGTGCCT AGAGAGTACT GGCGGGACCG GCTGCTGAAA CTGAAGGCCT GTGGCTTCAA
     3361 CACCGTGACC ACCTACGTGC CCTGGAATCT GCACGAGCCT GAGAGAGGCA AGTTCGACTT
     3421 CAGCGGCAAC CTGGACCTGG AAGCCTTTGT GCTGATGGCC GCTGAGATCG GACTGTGGGT
     3481 CATCCTGAGG CCTGGCCGGT ACATCTGTTC TGAGATGGAT CTGGGAGGCC TGCCTAGCTG
     3541 GCTGCTGCAG GATCCTAGAC TGCTGCTGAG AACCACCAAC AAGAGCTTCA TCGAGGCCGT
     3601 GGAAAAGTAC TTCGACCACC TGATCCCTAG AGTGATCCCC CTGCAGTATA GACAGGCCGG
     3661 ACCTGTGATT GCCGTGCAGG TCGAGAATGA GTACGGCTCC TTCAACAAGG ACAAGACCTA
     3721 CATGCCTTAC CTGCACAAGG CCCTGCTGCG GAGAGGAATT GTGGAACTGC TCCTGACCTC
     3781 CGACGGCGAG AAACACGTGC TGTCTGGACA CACAAAAGGC GTGCTGGCCG CCATCAATCT
     3841 GCAGAAGCTG CACCAGGACA CCTTCAACCA GCTGCACAAA GTGCAGCGGG ATAAGCCCCT
     3901 GCTGATCATG GAATACTGGG TCGGATGGTT CGACAGATGG GGCGACAAGC ACCACGTGAA
     3961 GGACGCCAAA GAAGTGGAAC ACGCCGTCAG CGAGTTCATT AAGTACGAGA TCAGCTTCAA
     4021 CGTGTACATG TTTCACGGCG GCACCAACTT CGGCTTCATG AATGGCGCCA CCTACTTTGG
     4081 CAAGCACAGC GGCATCGTGA CAAGCTACGA CTACGACGCC GTGCTGACAG AGGCCGGCGA
     4141 TTACACAGAG AAGTACCTGA AGCTCCAGAA ACTGTTCCAG AGCGTGTCCG CCACACCACT
     4201 GCCAAGAGTG CCAAAACTGC CTCCCAAGGC CGTGTACCCT CCTGTCAGAC CATCTCTGTA
     4261 CCTGCCTCTG TGGGATGCCC TGAGCTACCT GAATGAGCCT GTGCGGAGCA GACAGCCTGT
     4321 GAACATGGAA AATCTGCCCA TCAACAACGG CTCCGGCCAG TCTTATGGCC TGGTGCTGTA
     4381 CGAGAAGTCC ATCTGCTCTG GCGGCAGACT GAGAGCCCAT GCTCATGATG TGGCCCAGGT
     4441 GTTCCTGGAC GAGACAATGA TCGGCATCCT GAACGAGAAC AACAAAGATC TGCACATCCC
     4501 CGAGCTGCGG GACTGCAGAT ACCTGAGAAT CCTGGTGGAA AACCAGGGCC GCGTGAACTT
     4561 CTCCTGGCAG ATCCAGAATG AGCAGAAGGG CATCACCGGC TCCGTGTCCA TTAACAATAG
     4621 CAGCCTCGAG GGCTTCACCA TCTACTCCCT CGAAATGAAG ATGAGCTTCT TTGAGCGGCT
     4681 GAGAAGCGCC ACATGGAAGC CAGTGCCTGA TTCTCACCAG GGACCTGCCT TTTACTGCGG
     4741 CACACTGAAA GCTGGCCCCA GCCCTAAGGA TACCTTCCTG TCTCTGCTGA ACTGGAACTA
     4801 CGGCTTCGTG TTCATCAACG GCCGGAACCT GGGACGGTAC TGGAACATCG GACCCCAGAA
     4861 AACCCTGTAT CTCCCTGGCG TGTGGCTGCA CCCCGAGGAC AATGAAGTGA TCCTGTTTGA
     4921 GAAGATGATG AGCGGCAGCG ACATCAAGAG CACCGACAAG CCAACACTCg aaaacttgta
     4981 ctttcaaggc tCCAGCTTTC TTGTACAAAG TGGTATGAGC AAGGGCGAGG AACTGTTCAC
     5041 CGGCGTGGTG CCCATCCTGG TGGAACTGGA TGGCGACGTG AACGGCCACA AGTTCTCTGT
     5101 GCGGGGAGAG GGCGAAGGCG ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC
     5161 CACCGGCAAG CTGCCCGTGC CTTGGCCTAC CCTCGTGACC ACACTGACCT ACGGCGTGCA
     5221 GTGCTTCAGC AGATACCCCG ACCATATGAA GCGGCACGAC TTCTTCAAGA GCGCCATGCC
     5281 CGAGGGCTAC GTGCAGGAAC GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG
     5341 AGCCGAAGTG AAGTTCGAGG GCGACACCCT CGTGAACCGG ATCGAGCTGA AGGGCATCGA
     5401 TTTCAAAGAG GACGGCAACA TCCTGGGCCA CAAGCTGGAG TACAACTTCA ACAGCCACAA
     5461 CGTGTACATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA
     5521 CAACGTGGAA GATGGCAGCG TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG
     5581 AGATGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA
     5641 GGACCCCAAC GAGAAGCGGG ACCACATGGT GCTGCTGGAA TTCGTGACCG CCGCTGGCAT
     5701 CACCCACGGC ATGAGCGGCC TGAACGACAT CTTCGAGGCC CAGAAAATCG AGTGGCACGA
     5761 GCACCACCAT CATCACCATC ACCACTGATG ATTCGAAAAA TAATAAGGAT CCAATCAACC
     5821 TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC
     5881 GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT
     5941 CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT
     6001 TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG
     6061 CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC
     6121 GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC
     6181 TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT
     6241 TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC
     6301 GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGAGATCT
     6361 GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC
     6421 TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG
     6481 CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG
     6541 GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGACTCGA GTTAAGGGCG AATTGCCGAT
     6601 TAGGATCTTC CTAGAGCATG GCTACGTAGA TAAGTAGCAT GGCGGGTTAA TCATTAACTA
     6661 CAAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA
     6721 GGCCGGGCGA CCAAAGGTCG CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA
     6781 GCGAGCGCGC AGCCTTAATT AAGAGCTCAT GGCGCGCCTA GGCCTTGACG GCCTTCCGCC
     6841 AATTCGCCCT ATAGTGAGTC GTATTACGTC GCGCTCACTG GCCGTCGTTT TACAACGTCG
     6901 TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC
     6961 CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGAAACGCCC TTCCCAACAG TTGCGCAGCC
     7021 TGAATGGCGA ATGGGAGCGC CCTGTAGCGG CCACTCAACC CTATCTCGGT CTATTCTTTT
     7081 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA
     7141 AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAG
//

